By M. Cristina Vega
This ebook offers complicated expression applied sciences for the construction of protein complexes. considering the fact that complexes lie on the center of recent biology, the expression, purification, and characterization of huge quantities of fine quality protein complexes is essential for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and bug cells to complicated reconstitution from person subunits, this e-book deals invaluable insights and information for profitable protein expressionists.
Across a number of sections readers will realize latest possibilities for the creation of protein complexes in bacterial structures (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, vegetation and algae. advanced reconstitution from separately purified subunits or subcomplexes is mentioned as a complementary process. a final part introduces in brief many of the biophysical and structural characterization ideas for macromolecular complexes utilizing cutting-edge resolution scattering and nuclear magnetic resonance.
This paintings is a guided travel over essentially the most robust and winning protein expression applied sciences, with a spotlight on co-expression and high-throughput functions. it's addressed to everybody attracted to the creation and characterization of macromolecular complexes, from college scholars who wish an obtainable description of the most important co-expression platforms to researchers in biomedicine and the existence sciences looking for an updated survey of accessible technologies.
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Additional resources for Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology)
Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology) by M. Cristina Vega